Figure 1.

Stereospecificity of NPD1 bioactivity selectively upregulates BIRC3 expression. (a) BIRC1 to 8 in response to 600 μM H₂O₂/10 ng/ml TNF-α in the presence or absence of 100 nM NPD1 at 2, 4 and 6 h of treatment. (b) BIRC3 mRNA expression in response to 100 nM maresin 1, lipoxin-A4 and RvE1 along with NPD1 and its stereoisomers SS-NPD1 and RR-NPD1 and (c) in an siRNA dose-dependent curve. ARPE-19 cells were transfected with 0, 5, 10, 20, 50 and 100 pmol of siRNA per ml of culture media and treated with 400 μM H₂O₂/10 ng/ml TNF-α in the presence or absence of 100 nM NPD1. (i–vi) Structure of the lipid mediators used in (b). The bars represent the mean of three triplicates ± standard error. *P<0.05, NS=non-significant P-value. NPD1-treated samples=blue bars; OS+NPD1=light blue bars