Figure 6.

Effect of deletion and overexpression of engineering target genes in C. glutamicum strain NA2 on the production of putrescine (black bars) and N-acetylputrescine (grey bars). Genetic changes introduced to the chromosome of C. glutamicum NA2 and to plasmid pVWEx1-speC-argF₂₁ are highlighted in bold. Genes for feedback-resistant N-acetylglutamate kinase (argB^A49V/M54V), pyruvate carboxylase (pyc) and glyceraldehyde 3-phosphate dehydrogenase (gapA) were added to plasmid pVWEX1-speC-argF₂₁, the translational start codon exchange of the γ-glutamate kinase gene proB from ATG to TTG was introduced in the chromosome, while the spermi(di)ne N-acetyltransferase gene snaA and the regulatory gene cgmR were deleted. Cells were grown in CGXII medium with 20 g·L^-1 glucose and 1 mM IPTG. Means and standard errors of three independent cultivations are shown.