Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 15;29(12):1285–1297. doi: 10.1101/gad.264234.115

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Chen et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — S112 phosphorylation is required for 14-3-3 binding and cytoplasmic translocation of endogenous YAP induced by contact inhibition in cell culture or developmental Hippo signaling in intact tissues. (A) Coimmunoprecipitation assay. Cell lysates of wild-type and Yap^S112A^/S112A MEFs were immunoprecipitated with α-14-3-3 antibody and immunoblotted with α-YAP antibody. (B) Loss of cell contact-induced YAP translocation in the Yap^S112A^/S112A MEFs. Wild-type and Yap^S112A^/S112A MEFs grown at low or high density were immunostained for endogenous YAP (green) and nuclear dye DAPI (blue). Endogenous YAP shows nuclear-to-cytoplasmic translocation at high cell density in the wild-type cells but not in the Yap^S112A^/S112A cells. Bar, 50 μm. (C) Immunostaining of YAP in liver sections from wild-type and Yap^S112A mice. Note the more prominent nuclear localization of YAP in the Yap^S112A liver compared with the wild-type liver (arrows). Also note the overall decrease of YAP staining intensity in the Yap^S112A liver. Tissue sections were processed in parallel and stained under identical conditions. Bar, 50 μm. (D) Immunostaining of YAP in colon sections from wild-type and Yap^S112A mice. Note the decrease of overall YAP staining and the more prominent nuclear localization in the colonic epithelial cells in Yap^S112A mice compared with the wild-type mice (arrows). Tissue sections were processed and stained under identical conditions. Bar, 50 μm.