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. 2015 Jun 15;29(12):1285–1297. doi: 10.1101/gad.264234.115

Figure 5.

Figure 5.

Compensatory reduction of YAP protein levels in the YapS112A mice. (A) Decreased YAP protein levels in the YapS112A livers. Liver lysates of wild-type, YapS112A/+, and YapS112A/S112A mice (4 mo old) were probed with the indicated antibodies. The graphs show quantification of YAP protein and mRNA levels. Note the absence of S112 phosphorylation and the decrease in YAP protein but not mRNA levels. Values are means ± SD. n = 3. (*) P < 0.05, t-test. (B) Decreased YAP protein levels and increased YAPS366 phosphorylation in multiple organs of the YapS112A mice (2 mo old). Note that although the p-YAPS366 level was comparable between mutant and wild-type brains, the ratio of p-YAPS366 relative to total YAP was higher in the mutant due to the dramatic decrease of YAP protein level (see quantification in C).Values are means ± SD. n = 3. (C) Quantification of relative YAP protein level (left graph) and P-YAP (S366)/YAP ratio (right graph) in different organs of wild-type and YapS112A mice. Data are mean ± SD from three animals of each genotype. (*) P < 0.05, t-test. (D) Western blot analysis of cell lysates from the wild-type and YapS112A MEFs.