Figure 6.

Compensatory decrease of YAP stability and activation of the Hippo kinase cascade in the Yap^S112A mice. (A) Faster turnover of endogenous YAP^S112A protein compared with endogenous wild-type (WT) YAP protein. Confluent cultures of wild-type and Yap^S112A MEFs were treated with 50 µg/mL CHX for different periods of time and probed with the indicated antibodies. A representative blot is shown. YAP protein levels were quantified by the LI-COR Odyssey imaging system from three parallel experiments, normalized to actin, and arbitrarily set as 1 at time 0 in the graph shown at the right. Note that twice as much Yap^S112A cell lysates were loaded in each lane to adjust for the lower levels of YAP protein in the Yap^S112A cells (*). (B) Western blot analysis of liver protein lysates from wild-type and Yap^S112A mice. The graphs show quantification of YAP, TAZ, NF2, Mst1, Mst2, Lats1, Lats2, p-Lats, TEAD1-4, and CTGF levels in mutant livers relative to wild-type livers. Data are mean ± SD from three animals for each genotype. (*) P < 0.05, t-test.