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. 2015 Jun 15;29(12):1326–1340. doi: 10.1101/gad.261115.115

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© 2015 Chen et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 2. — SEC-MALS analysis of the hSpt2C–histone H3/H4 complex. (A) Analysis of histone H3/H4 (blue), hSpt2(571–685) (green), and H3/H4–hSpt2(571–685) (red) under 2 M NaCl conditions. Reyleigh ratio in continuous lines as a function of the elution volume. The calculated molecular mass is shown as a dashed line in the corresponding color with the secondary scale at the right. (B) Analysis of histone H3/H4 (blue), SUMO-tagged hSpt2(571–685) (green), H3–H4–hSpt2(571–685)SUMO (red) under 2 M NaCl conditions. Reyleigh ratio in continuous lines as a function of the elution volume. The calculated molecular mass is shown as a dashed line in the corresponding color with the secondary scale at the right. (C) Tables of mass measurements by SEC-MALS.