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. 2015 Jun 11;11(8):923–934. doi: 10.7150/ijbs.10896

Figure 4.

Figure 4

Inhibition of protein levels of CSC-related molecules and Hsps, and activation of pro-apoptotic cascade by amurensin G, and suppression of mRNA levels of 17-AAG-mediated induction of Hsp70/Hsp27 and ABCB1/ABCG2 genes by amurensin G in CD44high K562 cells. A: Cells were pretreated with amurensin G (5 μg/ml) for 6 h, and treated with 17-AAG (1 or 10 μM) for additional 24 h, and the indicated proteins were detected by western blot analysis. CF; cleavage fragments. B: Relative mRNA levels of Hsp70/Hsp27 and ABCB1/ABCG2 genes in the cells treated with 17-AAG alone and co-treated with 17-AAG and amurensin G were determined by real time RT-PCR. Each bar represented the mean ± S.D. of triplicate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control, #p < 0.05, ##p < 0.01 and ###p < 0.001, 17-AAG alone vs. combination with amurensin G.