Figure 4.

The Na⁺/K⁺ ATPase is mis-located to the apical surface of pronephric duct in yap morphants. (A-B) Whole-mount staining shows that the inner diameter of the enlarged duct indicated by aPKC is about fourfold of the control, however, the change of the outer diameter indicated with α-6F is not that dramatic. (C-D') The cross section view demonstrates increased cell number surrounding the dilated duct compared with the control duct (white dashed circles represent the cross sections of the ducts), the baso-lateral staining of Na⁺/K⁺ ATPase is not obvious in a part of enlarged tubules (Fig.4D'; red arrow), and even is mis-targeted to the apical surface adjacent to aPKC (D'; arrowhead). (C' and D') Arrows indicate basal surface and asterisks indicate lateral surface. (A-B) Bar: 10 μm. (C-D) Bar: 5 μm.