Fig 6.

Overexpression of FGF9 promotes the two-way communication between human PCa and stromal cells. A. Real time RT-PCR analyses for Fgf9 expression in LNCaP cells. B. The indicated LNCaP cells were cultured with or without HPS-19I human prostate stromal cells in transwell assay. LNCaP cells migrating through the membrane were stained with hematoxylin (panels a-d), and the average numbers of cells from triplicate samples were calculated and presented as mean ± sd (panel e). C. Real time RT-PCR analysis of LNCaP cells with or without HPS-19I cell coculture (a), with or without FGF9 treatment (b), or HPS-19I cells cocultured with FGF9^high or FGF9^low cells (c). FGF9^high, high FGF9-expressing LNCaP cells; FGF9^low, low FGF9-expressing LNCaP cells; HPS, HPS-19I human prostate stromal cells; SMA, α-smooth muscle actin.