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. 2015 Jun 11;11(8):970–981. doi: 10.7150/ijbs.11635

Figure 2.

Figure 2

DT-13 suppressed the expression of ICAM-1 and VCAM-1 in TNF-α-activated HUVECs. (A-B). HUVECs were pretreated with DT-13 (0.01, 0.1, and 1 μM) for 1 h followed by TNF-α (10 ng/mL) exposure for 4 h. ICAM-1(A) and VCAM-1(B) surface expression were detected by flow cytometry. (C-D). The expression of ICAM-1(C) and VCAM-1(D) was detected by Western blotting. Representative blots normalized to GAPDH expression are presented. The data represent the mean ± SD from three experiments. ##P<0.01 vs. the control group; * P<0.05, ** P<0.01 vs. the TNF-α group.