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. 2015 Jun 11;11(8):982–991. doi: 10.7150/ijbs.12096

Table 1.

In vitro assays commonly used to screen antioxidant activity of plants.

Sr. No. Assay Mechanism References
1 β-Carotene or crocin-bleaching assay Hydrogen atom transfer Karadag et al. 63
2 ORAC (oxygen radical absorbance capacity) Hydrogen atom transfer Ou et al. 64
3 IOU (inhibited oxygen uptake) Hydrogen atom transfer Karadag et al. 63
4 LPIC (lipid peroxidation inhibition capacity) assay Hydrogen atom transfer Karadag et al. 63
5 TRAP (total radical trapping antioxidant parameter) Hydrogen atom transfer Karadag et al. 63
6 Copper reduction assay Single electron transfer Huang et al. 65
7 FRAP (ferric reducing antioxidant power assay Single electron transfer Huang et al. 65
8 Total phenolic content assay by Folin-Ciocalteu reagent Single electron transfer Karadag et al. 63
9 ABTS ({2,2' - azinobis-(3-ethyl-benzothiazoline-6-sulphonic acid)}) assay Both hydrogen atom and single electron transfer Huang et al. 65
10 DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay Both hydrogen atom and single electron transfer Huang et al. 65