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. 2015 Jul 8;5:12003. doi: 10.1038/srep12003

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(a) Flow cytometric analysis for annexin V and PI in glioma cells treated with compound 331 (20 μM, 48 h) or transfection wtih CDC20 overexpressing plasmid (24 h before treated with compound 331). Percentage of annexin V-positive and PI-negative cells (bottom right) were indicated. (b) Proteolytic activities of caspase-3/7 induced by compound 331 (20 μM, 48 h) and rescued by overexpression of CDC20 (transfected 24 h before treatment with compound 331). Data represents the mean ± S.E.M. of three independent experiments. **p < 0.01 compared with the control groups.