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. 2015 Jul 8;5:11724. doi: 10.1038/srep11724

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) catRAPID fragments based prediction of interaction between HOTAIR and HPV16 E7 and maps HOTAIR interaction domains to nts 1–770 (5′-end) and nts 1700–2370 (3′-end). (b,c) catRAPID graphic output confirming the interaction of HPV16 E7 to the 5′ and 3′ ends of HOTAIR transcript as predicted by catRAPID fragments. (d) qRT-PCR results shows HOTAIR is significantly enriched with the SUZ12 antibody compared to IgG (control antibody) in untransfected C33A cells while a significant enrichment of HOTAIR was found both for HPV16 E7 and SUZ12 antibody in HPV16 E7 expressing C33A cells although lower for SUZ12.