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. 2015 Jul 8;5:11762. doi: 10.1038/srep11762

Figure 5. Glycosylation modification of Gda1p is not necessary for yeast sporulation.

Figure 5

(a) Schematic representation of the mutant glycosylation sites, which included GDA1 (Gda1p 1–518aa), N41D (Gda1p N41D), N280D (Gda1p N280D), N335D (Gda1p N335D), and N41D/N280D/N335D (Gda1p. N41D/N280D/N335D). (b) Glycosylation site mutations of GDA1 were not necessary for yeast sporulation. The WT strain harboring empty vector and the gda1Δ strains harboring the empty vector or GDA1, N41D, N280D, N335D, N41D/N280D/N335D under the control of its own promoter were incubated in sporulation medium for 24 hrs. Sporulation efficiency was determined by staining with DAPI. (c) Microscopic observation of the WT strain harboring empty vector and the gda1Δ strains harboring either the empty vector, WT GDA1 or glycosylation site mutants under the control of their own promoter after sporulation induction for 24 hrs.