Figure 1. High-throughput sphere formation microfluidic chip.

(a) Schematic showing cells settling into a microwell during loading. (b) Schematic showing cell aggregation and sphere formation 1 day after cell loading. (c) 3D schematic view of the microfluidic chip. (d) Enlarged 3D schematic of micro-wells. (e) The fabricated device that contains 1,024 micro-wells within a core area of 2 cm by 2 cm. The cells are loaded in the inlet (top right) and then flow through the microwell array to the outlet (bottom left).