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. 2015 Jul 8;5:11924. doi: 10.1038/srep11924

Figure 5. EMT phenotypes were reversed by blocking TGFβ/Smad signaling in the CAF-CM treated bladder cancer cells.

Figure 5

A,B: The protein levels of E-cadherin and Vimentin in the CAF-CM treated 5637 and J82 cells upon the blocking of a neutralizing antibody (A) or a TGFβR1 inhibitor, SB-431542, (B) by immunoblotting. C,D: The mRNA levels of mesenchymal markers and EMT-associated transcription factors in the CAF-CM treated 5637 and J82 cells upon the blocking of a neutralizing antibody (C) or a TGFβR1 inhibitor (SB-431542) (D) by qRT-PCR. Cell migration (E) and invasion ability (F) in the CAF-CM treated 5637, T24 and J82 cells upon the blocking of a neutralizing antibody by the Transwell cell migration/invasion assay. *P < 0.05, **P < 0.01, ***P < 0.001.