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. 2015 Jul 8;5:12030. doi: 10.1038/srep12030

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Three tissue portions were isolated from rat brains at 48 h post-ischemia: infarcted tissue (lesion), as delimited by TTC staining; a 2-mm strip around the infarct region (peri-infarct); and control tissue from the contra-lateral hemisphere. The protein content from each portion was extracted in three different fractions (membrane, soluble and insoluble). The samples were analysed 2D-PAGE and COFRADIC proteomics. Proteins with significant differences of expression between the tissue portions (>3-fold, p < 0.05) were further studied by western blotting (WB) and immunohistochemistry (IHC). Data were combined to define suitable molecular targets of the peri-infarct tissue.