Figure 2. FGF21 improves hepatic metabolic profile in Zucker rats.

Livers from Zucker rats administered 1.32 mg/kg/day native FGF21 for two weeks were harvested. a) representative western blots for precursor and mature SREBP1, b) incorporation of 2-14C-acetate in ex vivo livers, n = 6/group, c) western blots for P-AMPK (Thr 172) and Tubulin, d) 14C incorporation into CO₂ from ex vivo liver slices, n = 6/group. e) Acute insulin response in Zuckers administered 1.32 mg/kg/day native FGF21 for two weeks. Western blots for P-Akt and total Akt from the indicated tissues. In Figures a and c, each lane represents a different animal. All gels reported in a), c) and e) were run under the same experimental conditions on the same gels respectively for each of these proteins. In a) each lane represents liver lysates from a separate animal, 4 administered PBS and 4 administered FGF21. All samples were run in the order shown in the figure. In c) data is shown for 3 animals administered PBS and 3 animals administered FGF21. Each protein was run on its own gel. Dotted line indicates where blots were cropped. In e) data is shown for 3 animals administered PBS and 3 administered FGF21. All samples were run in the order shown in the gels. Representative total Akt blot is from liver. Each protein was run on its own gel. Data represented as Mean ± SEM. * p < 0.05 by paired t-test.