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. 2015 Jul 8;11:38. doi: 10.1186/s13007-015-0081-7

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© Huang and Yu. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Development of symplastic connection between stocks and scions. Arabidopsis gl1-1 scions were grafted onto wild-type stocks under LD conditions. The 5(6)-carboxyfluorescein diacetate dye (green fluorescence) was introduced onto leaves (indicated by arrows) of wild-type stocks at different times after grafting. The images (left panels) and florescence images (right panels) were taken at 4 days (a, b), 5 days (c, d), 6 days (e, f), and 8 days (g, h) after grafting. Note green fluorescent signals (or yellow colors when merged with red florescence) in the vasculature of gl1-1 scions at 6 or 8 days after grafting but not 4 or 5 days after grafting. The red colors in florescence images represents the auto-florescence of chlorophyll. The glabrous leaves of scions are indicated by white asterisks (left panels).