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10 μM TFEα/β-His were incubated together with 10 μM recombinant RNAP clamp and the sample was resolved gel filtration. The presence of RNAP clamp and TFEα/β in the fractions was determined by SDS-PAGE and silver staining. The position of peaks for gel filtration marker proteins is indicated on top. For experiments with TFEα/β ΔWH the contrast was enhanced for the lower part of the gel in order to visualize TFEβ ΔWH.
