Figure 1.

Query of sphingolipid metabolism and S1P signaling receptor genes from low, median and highly metastatic MDA-MB-231 sublines. Previously described microarray data obtained from various MDA-MB-231 sublines of varying metastatic capacity were queried for predictive value to the expression of genes involved in sphingolipid/S1P metabolism and signaling. Technical details of these studies have been described previously.24 (a) Displays a simplified schematic of sphingolipid/S1P metabolism and S1PR receptor genes (italicized) chosen for this retrospective analysis. TGFβ1-induced SPHK1 expression was identified and provided significant predictive value between low, median and high metastatic behavior of the various MDA-MB-231 (SCP-derived) sublines in mouse metastasis models (b). **P<0.01 (Student's t-test, low vs median/high).