Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 2;10:4279–4291. doi: 10.2147/IJN.S85095

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Li et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Detection of VEGF expression.

Notes: (A) Suppression of VEGF mRNA levels determined by quantitative real-time PCR. (B) The secretion of VEGF protein in culture media tested by human VEGF ELISA kit; both of the experiments were executed at 72 hours after transfection with Fe₃O₄@SiO₂/PEI (NPs/PEI), Fe₃O₄@SiO₂/PEI/Sc shRNA (NPs/PEI/Sc shRNA), or Fe₃O₄@SiO₂/PEI/VEGF shRNA (NPs/PEI/VEGF shRNA). All the NP concentrations are 60 μg/mL. For the NPs/PEI/Sc shRNA and NPs/PEI/VEGF shRNA, the weight ratio of NPs/PEI to Sc shRNA or VEGF shRNA is 40:1. *P<0.05 compared with NPs/PEI or NPs/PEI/Sc shRNA.

Abbreviations: mRNA, messenger RNA; NPs, nanoparticles; PEI, polyethylenimine; Sc, scrambled; shRNA, small hairpin RNA; PCR, polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay.