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Agarose gel electrophoresis retardation assays.
Notes: (A) Fe3O4@SiO2:VEGF shRNA and (C) Fe3O4@SiO2/PEI:VEGF shRNA at different weight ratios (5:1, 10:1, 20:1, 30:1, and 40:1) in water; and (B) Fe3O4@SiO2:VEGF shRNA and (D) Fe3O4@SiO2/PEI:VEGF shRNA at different weight ratios (5:1, 10:1, 20:1, 30:1, and 40:1) in 0.9% NaCl.
Abbreviations: shRNA, small hairpin RNA; PEI, polyethylenimine.
