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. 2015 Jul 1;56(8):4198–4210. doi: 10.1167/iovs.15-16557

Figure 2.

Figure 2

Aldose reductase inhibitors or AR knockdown decreases TGF-β2–dependent SMADs phosphorylation. Human lens epithelial B3 cells were incubated with TGF-β2 (5 ng/mL) for indicated times (A). Human lens epithelial B3 cells were pretreated with vehicle (V) or Sorbinil (S, 10 μM) for 30 minutes followed by TGF-β2 (5 ng/mL) for 30 minutes (B). Cells were also transfected with control or AR siRNA for 72 hours followed by treatment with TGF-β2 for 30 minutes (C). In all cases, the fold-activation of p-SMAD2 and p-SMAD3 were normalized to total SMAD2 and SMAD3, respectively. Fold change is compared with vehicle in the TGF-β2 group. The efficiency of AR knockdown was examined by probing with AR antibody. Changes in the level of proteins of interest were analyzed for statistical significance and are displayed in graphical form as mean values ± SEM (n = 3). *P < 0.05.