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. 2015 Jul 1;56(8):4198–4210. doi: 10.1167/iovs.15-16557

Figure 3.

Figure 3

Aldose reductase inhibitors or AR knockdown limit MMP-2 and MMP-9 activation by TGF-β2. Human lens epithelial B3 cells were pretreated with vehicle (V) or Sorbinil (S, 10 μM) for 30 minutes followed by TGF-β2 (5 ng/mL) exposure for 24 hours (A). Cells were further transfected with control (siCtrl) or AR siRNA (siAR) for 72 hours and followed by TGF-β2 exposure (B). Pro-MMP-2, -9 and active MMP-2, -9 secreted in medium were detected by gelatin zymography (A, B). Fold change of pro-MMPs is compared with vehicle or siCtrl in no TGF-β group. Changes in the level of proteins of interest were analyzed for statistical significance and are displayed in graphical form as mean values ± SEM (n = 3). *P < 0.05; **P < 0.01.