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Principal scheme of a three-input deoxyribozyme AND gate (3iAND). A) Strands 3iANDa and 3iANDb of the gate are dissociated in the absence of inputs. Dashed lines indicate the input-recognition fragments of the strands. B) Catalytic Dz complex formed in the presence of all three inputs (I1, OP1, and I2). The Dz catalytic core cleaves the fluorophore- and quencher-labelled F substrate and increases fluorescent signal. Note that OP1 is not fluorescently labelled.
