Table 2. Three formulations of the Luminol-H2O2-HRP-enhancer CL system.
4-IMP | 4-IOP | HIOP | |
---|---|---|---|
DMF (%) | 0.20 | 3.2 | 3.2 |
Luminol (mM) | 0.63 | 0.63 | 0.63 |
H2O2 (mM) | 2 | 4 | 4 |
Enhancer (mM) | 1 | 0.5 | 1 |
pH and Ionic (M) | 9.0, 0.01 | 9.0, 0.01 | 9.0, 0.01 |
HRP (ng/ml) | 0~6 | 6~25 | 25~80 |