Fig 2. Phylogenetic tree showing relationships between Nearctic Myotis (sensu [58]), and the presence of three distinct clades within M. lucifugus, based on maximum likelihood analysis of partial COI sequences in PhyML 3.0.

A member of the Neotropical Myotis clade (M. austroriparius) was included as the outgroup. Leaves are collapsed to highlight well-supported clades, and the vertical dimension of the triangles is proportional to the number of samples included. SH-like branch support values are provided for all major clades. Clades containing M. lucifugus are designated by the specific abbreviation followed by the subspecies name (e.g., M. l. lucifugus refers to the nominal subspecies). Note that one clade (including M. l. carissima) also contains members of other species (including M. evotis, M. keenii, and M. thysanodes) as previously described [32].