Figure 7. The Activity of a REV-ERB Agonist is Suppressed by Treatment with Lithium.

A, Treatment with lithium results in suppression of the wakefulness inducing effects of SR9009. Mice were maintained on either water or water with lithium chloride as described in the methods and vigilance states were monitored by EEG. Vehicle injection or SR9009 injection (100 mg kg⁻¹, i.p.) is indicated. N=6 mice per group. B, Treatment with lithium suppresses the anxiolytic activity of SR9011 in a marble burying assay. Mice were maintained on either water or water with lithium chloride and treated with vehicle or SR9011 (100 mg kg⁻¹ b.i.d., i.p.) as described in the methods. n=8 mice per group. Within the EEG studies (wakefulness, SWS and REM sleep), potential differences between treatments were assessed by repeated measure two-way ANOVA followed by Bonferroni post hoc test. In the marble burying assay differences between treatment groups were assessed by a two tailed t test (Student’s) with significance *,P < 0.05.