Figure 9. SR10067 Alters Sleep Architecture and Anxiety-like Behavior.

A, Actograms from wheel running cages demonstrating the effect of SR10067 injection (red bars, i.p. various concentrations) on wheel running activity in mice. B, Assessment of the dose-dependence of inhibition of wheel running activity in mice during the entire dark phase following administration of SR10067. n=6 to 8 mice per group. C, Assessment of the effect of SR10067 on wakefulness in mice. Wakefulness, SWS and REM sleep was monitored by EEG as indicated in Figs. 1 & 2. Mice were injected with SR10067 (30 mg kg⁻¹, i.p.) or vehicle at ZT6. n=8 mice. D, Locomotor telemetry data indicating an increase in movement of mice during the period 2h after ZT6 injection of SR10067 (30 mg kg⁻¹). n=6 mice. E, Results from the marble burying assay demonstrating that SR10067 dose-dependently reduces anxiety like behavior in the marble burying assay. n=8 mice. Values are mean ± SEM. In panel D differences between treatment groups (vehicle vs. SR) were assessed by a two tailed t test (Student’s) with significance *P < 0.05. In panels B and E, differences between groups were assessed using one-way ANOVA followed by Tukey’s post hoc test with significance *P<0.05. In panel C, potential differences between treatments were assessed by repeated measure two-way ANOVA followed by Bonferroni post hoc test with significance *P < 0.05.