Figure 5. MRC1 Mutation Exacerbates ETI Cell Cycle and Lifespan Phenotypes but not Senescence or Telomere Shortening Rates.

Mother cell budding profiles for mrc1^AQ(A) and tlc1Δ mrc1^AQ (B). (C) mrc1^AQ (n=40) strain lifespan does not differ from WT. (D) tlc1Δmrc1^AQ (n=90) mutation worsens the lifespan reduction caused by ETI mutations in mother cells (p-value < 2e-7, compared with tlc1Δ). (E) ETI and ETI mrc1^AQ mutants displayed similar rates of senescence when passaged on solid media. (F) Southern blot analysis of telomeric DNA restriction fragment lengths of cells taken from plates after serial streaks shown in (E).