View of a 3-fold pore in wt BfrB and mutants. The top row shows cross sectional views of the 3-fold pores in the as-isolated structures. The middle row depicts an identical cross-section view of the 3-fold pores in the Fe-soaked structures, with Fe3f iron ions rendered as orange spheres, sulfate ions as green (S) and red (O) spheres, and structured water as yellow spheres. The bottom row depicts the 3-fold pores viewed from the interior cavity, to illustrate the network of interactions involving iron, sulfate and protein side chains.