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. 2008 Jul 9;13(6):1136–1148. doi: 10.1111/j.1582-4934.2008.00394.x

Figure 6.

Figure 6

Effects of the selective inhibitors of MAPK on DNA binding activities of SP1 and AP1 in HUVEC-12 cells stimulated with LPC. The nuclear extracts from HUVEC-12 cells treated without or with 40 μMol/l of LPC for 2 hrs were used for EMSA to detect effect of the specific inhibitors of MAPKs on SP1 and AP1 DNA binding activity by stimulation of LPC. Lane 1, free probe; lane 2, treated without LPC as a control; lanes 3∼4 (A, B), 50- and 100-fold molar excess of cold probe of SP1 was used to determine SP1-specific DNA binding; lane 3 (C, D) 100-fold molar excess of cold probe of AP1 was done; lanes 5 (A, B) and 4 (C, D), treated with LPC as a stimulation group; lanes 6∼8 (A, B) and 5∼7 (C, D), pretreated with PD98059 (50 μMol/l), curcumin (30 μMol/l) and SB203580 (15 μMol/l), respectively, for 1.5 hrs before the stimulation of LPC (40 μMol/l). The data represent means ± S.D. of 3 independent experiments. *P< 0.05, **P< 0.01, compared with the control; ##P< 0.01, compared with the stimulation group.