Fig 4.

In vitro Matrigel invasion assay using U138MG and U251MG cells. Treatment (72 hrs) of cells: transfection with a plasmid vector expressing scrambled siRNA (treated control), 100 nM taxol, transfection with a plasmid vector expressing Bcl-2 siRNA and taxol + Bcl-2 siRNA. Invasion assays were carried out in 12-well transwell inserts of polycarbonate filters. After 48 hrs incubation at 37°C in a CO₂ incubator, the membranes were collected and stained with HEMA. The number of cells that migrated to the undersurface of the membrane were examined under a microscope, counted and photographed. (A) The changes in capability of invasion of U138MG cells after the treatments. (B) Quantitative evaluation of invading U138MG cells. (C) The changes in capability of invasion of U251MG cells after the treatments. (D) Quantitative evaluation of invading U251MG cells. The quantitative data are presented as mean ± S.D. of cells from 10 randomly selected microscopic fields from three independent wells (*P < 0.001 when compared with the scrambled siRNA treatment mean values and #P < 0.001 when compared with taxol or Bcl-2 siRNA treatment mean values).