Figure 4.
Activation of NF-κB in pancreatic acinar cells under basal conditions and under stimulation with pancreatitis-associated ascitic fluid (PAAF, 20% v/v) in the absence or presence of dexamethasone (Dx, 10−7 M) and N-acetylcysteine (NAC, 30 mM). (A) Representative Western blot of IκBα and the mean values of five experiments are shown. Band intensity was determined by densitometry. (B) p65 nuclear levels (NF-κB-DNA binding). Results are mean ± S.E.M. ANOVA followed by Dunnett’s test showed significant differences versus non-PAAF-stimulated cells (basal conditions) (**P < 0.01) and versus PAAF-stimulated cells (♦♦P < 0.01).