Figure 4.

Anx A5 promoter activity of activated macrophages. Macrophages were obtained from the peritoneal lavage of Anx A5 KO mice. Following, the macrophages were cultured for 24 hrs in medium without (w/o) or with LPS. The peritoneal macrophages were stained with 5-bromo-4-chloro-3-indoxyl beta-D-galactoside (X-Gal) for b-galactosidase activity (A), which represents the promoter activity of the Anx A5 gene. The X-Gal staining of macrophages (deep black areas) clearly demonstrates the expression of Anx A5 within the macrophage population (arrows). The b-galactosidase activity was also quantified by staining the Anx A5 knock out macrophages with fluorescein di-β -D-galactopyranoside (FDG) reagent. F4/80 positive macrophages displayed a 4.3-fold higher ß-Gal activity after stimulation with LPS. One out of three representative set of experiments is shown. KO: knock out; WT: wild type; LPS: lipopolysac-charide