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. 2015 Mar 24;6(11):8552–8566. doi: 10.18632/oncotarget.3298

Figure 5. Influence of p38MAPK inhibition on expression of senescence markers in Nd2-mutant mouse fibroblasts.

Figure 5

A. Primary fibroblasts of 3-month-old mice were treated with doxorubicin and SB203580 alone or in combination. Cellular proliferation was measured by BrdU incorporation assay on day 4. B. Primary fibroblasts from skin of 3-month-old mice were treated with doxorubicin and SB203580 alone or in combination. ATP levels were measured on day 4. C. After treatment of fibroblasts from skin of 3-month-old mice with doxorubicin and SB203580 alone or in combination, cell supernatants were collected and analysed by ELISA for IL-6. The concentration of doxorubicin was 250 nM, exposure time was 1 h; SB concentration was 10 μM for continuous treatment. n = 3. Data are given as mean + SEM. *p < 0.05; **p < 0.01; ***p < 0.001.