Figure 4. Overexpression of DACH1 suppresses cell growth and migration of HCC cell line in vitro.

Hep3B cells stably expressing DACH1 were confirmed by (A) immunofluorescent stain and (B) Western blotting. Representative examples of colonies and quantification of data were shown as mean ± SE (C). Cellular proliferation was measured by cell number counting (D) and CCK-8 value (E), with the data displayed as mean ± SE. Hep3B cells were assessed for migration ability by wound closure (F) and the data shown as mean ± SE (G). Transwell assays were employed to examine the invasion (H) with results shown as mean ± SE (I).