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. 2015 Apr 7;6(11):8676–8686. doi: 10.18632/oncotarget.3254

Figure 1. MiR-129-5p directly suppresses YAP and TAZ.

Figure 1

(A) Predicted miR-129-5p target sequence in 3′ UTRs of YAP and TAZ. Target sequences of YAP- and TAZ-3′ UTR were mutated. (B) Western blots of YAP and TAZ expression. α-Tubulin served as the loading control. (C) Luciferase assay of cells transfected with pGL3-YAP-3′UTR, pGL3-TAZ-3′UTR, pGL3-YAP-3′UTR-mut or pGL3-TAZ-3′UTR-mut reporter with miR-129-5p mimic, antagomiR-129-5p, mimic control or antagomiR control. (D) MiRNP IP assay showing the association between miR-129-5p with YAP and TAZ transcripts. GAPDH and 5s rRNA served as the negative controls. Each bar represents the mean ± SD of three independent experiments. *p < 0.05.