Figure 2. MiR-129-5p inactivates the Hippo signaling pathway.
(A) Western blotting of nuclear YAP and TAZ expression. The nuclear protein p84 was used as a nuclear protein marker. (B) TEAD transcriptional activity was assessed in the indicated cell lines by TEAD dependent promoter-driven firefly luciferase reporter construct. (C and D) Real-time PCR analysis of CTGF and Cyclin A in indicated cells. Transcript levels were normalized to GAPDH expression. Each bar represents the mean ± SD of three independent experiments. *p < 0.05.