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. 2015 Apr 2;6(11):8709–8721. doi: 10.18632/oncotarget.3325

Figure 5. CD44ICD interacts with the stemness factors, Nanog, Sox2, and Oct4 and regulates the nuclear-localization.

Figure 5

(A) MDA-MB-231 cells were transfected with CD44ICD. The interaction between CD44 and Nanog, Sox2, and Oct4 were detected with an immunoprecipitation assay described in “materials and methods”. (B) HEK293 cells were transfected with CD44ICD or the truncated mutant constructs, ICD_ΔN17, ICD_ΔN35, ICD_ΔC19, and co-transfected with Sox2 and Oct4 expression vectors for 36 hr as described in “materials methods”. Their interaction was detected with an immunoprecipitation assay. (C) Endogenous CD44 stable knockdown MDA-MB-231 and MCF-7 cells were transfected with the control and CD44ICD and C-terminal region truncated CD44 vectors. The changes in the localization of CD44ICD and stemness factors were detected by western blot. GAPDH and Lamin A/C were used as loading controls.