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. 2015 Mar 6;6(11):8722–8735. doi: 10.18632/oncotarget.2726

Figure 5. GANT61/PI103-mediated BAX/BAK activation is required for apoptosis.

Figure 5

(A) RD, TE381.T and RH30 cells were treated with 1 μM PI103 and/or GANT61 (RD 6 μM; TE381.T 8 μM; RH30 8 μM) and activation of BAK and BAX was determined after 24 hours by immunoprecipitation using active conformation-specific antibodies; representative blots are shown. (B) cells were transfected with non-silencing siRNA (siControl) or siRNA targeting BAK, treated with 1 μM PI103 and GANT61 (RD 6 μM; TE381.T 8 μM; RH30 8 μM) for 48 hours and apoptosis was determined by DNA fragmentation of PI-stained nuclei using flow cytometry. Mean + S.D. of three independent experiments performed in triplicate are shown; *p < 0.05; **p < 0.01; ns: not significant.