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. 2015 Mar 12;6(11):8807–8821. doi: 10.18632/oncotarget.3556

Figure 7. Increased expression of AGR2 is associated with Tgfbr1 deletion.

Figure 7

(A) Immunohistochemistry of AGR2 in tongue of the wild type (WT) mice, the tumor of Pten conditional knock out mice, Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice (Scale bar+25μm); (B) Quantitative of histoscore of AGR2 in wild type mice, Pten conditional knock out mice, Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice. Expression of AGR2 in Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice was significantly higher than the wild type mice (P < 0.001); (C) Quantitative Real-time PCR revealed the mRNA level of AGR2 in Tgfbr1 conditional knock out mice and Pten/Tgfbr1 conditional knock out mice was significantly increased when compared with the wild type mice; (D) Western blot analysis of AGR2 48h after knocking down PTEN, TGFBR1, and combined TGFBR1/PTEN by using siRNA; (E) Quantitative analysis showed the protein level of AGR2 in TGFBR1 siRNA group and TGFBR1/PTEN combined siRNA group were significantly higher than the control group (P < 0.001). Mean±SEM, ***, P<0.001.

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