Figure 5. Dimerization impaired CTED mutants fail to induce tumorigenesis in xenograft mouse model.

(A) The images are representative of tumor resection from xenografted mice harboring NIH-3T3 cells stably expressing either CTED4, CTED5, or CTED8 with or without receiver mutation (I941R) or activator mutation (L704N). (B) The growth of mouse tumors driven by CTED4, CTED5, and CTED8 mutants were significantly suppressed by dimerization impairing L704N and I941R mutations. Tumor size was measured 3 times per week, and the volume was determined according to the formula V=ab²/2, where a and b were tumor length and width, respectively (n=9 for each mutant group, mean ± SD). (C) Lysates prepared from the xenograft tumors harboring CTED mutants with or without dimerization impairing mutation were subjected to immunoblotting with antibodies against total or phospho-specific EGFR, Akt, and Stat3.