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. 2015 Mar 12;6(11):8914–8928. doi: 10.18632/oncotarget.3563

Figure 4. miR-203 targets SNAI2.

Figure 4

(A) Sequence alignment of human miR-203 with 3′-UTR of SNAI2. The seed sequence of miR-203 (top) matches 3′-UTR of SNAI2 (middle). Bottom, mutation of the 3′-UTR of SNAI2 in mutant luciferase reporter construct. (B) qRT-PCR shows that SNAI2 is upregulated in U251AR and U87AR cells. (C, D) qRT-PCR and western blotting assay of U251AR or U87 cells transfected with miR-203 or anti-miR-203, respectively. (E) Dual luciferase assay was performed in U251AR cells transfected with luciferase construct alone or cotransfected with miR-203 and anti-miR-203. Firefly luciferase construct containing mutant target site of the SNAI2 3′-UTR was generated and transfected as indicated. Firefly luciferase activity was normalised to Renilla luciferase activity for each sample. (F) Pearson correlation analysis shows a significant inverse correlation between miR-203 expression level and SNAI2 mRNA level in human GBM specimens. The results shown represent the mean±s.d. from three independent experiments. **P < 0.01.