Figure 7. Knockdown of EphB1 receptor enhances the percentage of cells in G1 phase in irradiated DAOY cells.
DAOY cells were transiently transfected with non-specific siRNA (Ns-siRNA) or EphB1-targeting siRNA and irradiated with 10 Gy at 24 h post-transfection. At 24, 48, and 72 h post-radiation, cell cycle profiles were assessed by flow cytometry. Data points represent the percentage of G1 cells in EphB1-siRNA treated group (normalized to Ns-siRNA treated group) in the absence and presence of ionizing radiation. Data shown are average ± standard error for n ≥ 2 independent experiments. The percentage of EphB1-siRNA treated cells in G1 phase of cell cycle at different time-points are indicated in the tabular format.