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. 2015 Mar 27;6(11):8947–8959. doi: 10.18632/oncotarget.3250

Figure 2. Increased expression of CD54 and MHC class I on OSCSCs differentiated with supernatants from IL-2+anti-CD16mAb treated NK cells.

Figure 2

OSCSCs were treated with NK supernatants in the presence and absence of anti-TNF-α and anti-IFN-γ antibodies as described in Figure 1A and the surface expression of CD54 and MHC Class 1 on untreated and NK supernatant treated OSCSCs were assessed after 4 days of differentiation. After differentiation, OSCSCs were washed and cultured in normal culture medium without the addition of NK supernatants for a period of 2 days, 6 days and 12 days. (A) Surface expression of CD54 and MHC Class I at each time point was assessed after PE conjugated antibody staining followed by flow cytometric analysis. Isotype control antibodies were used as controls. The numbers on the right hand corner are the percentages and the mean channel fluorescence intensities in each histogram. The ratios of MHC class I (B) or CD54 (C) at days 0, 2, 6 and 12 were determined by using the mean channel fluorescence of the IL-2+anti-CD16mAb treated NK supernatant differentiated OSCSCs to the mean channel fluorescence of the untreated OSCSCs.