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. 2015 Mar 13;6(11):9002–9017. doi: 10.18632/oncotarget.3282

Figure 3. Down-regulation of NOXA significantly attenuates apoptosis and inhibition of liver-cancer-cell proliferation induced by MLN4924+CQ.

Figure 3

(A-B) Down-regulation of NOXA significantly attenuated apoptosis induced by MLN4924+CQ. HepG2 and Huh7 cells were transfected with a control siRNA or NOXA siRNA and were then treated with MLN4924 (0.33 μM), CQ (10 μM), or both for 72 hours. Apoptosis induction was quantified by Annexin V-FITC/PI double-staining analysis (A) or caspase 3 activity analysis by FACS (B). (C) Knockdown efficiency and the expression of cleaved PARP were assessed by immunoblotting. (D) Cell viability was measured using the ATPLite assay (*P < 0.05; **P < 0.01, n = 3).