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. 2015 Mar 5;6(11):9031–9044. doi: 10.18632/oncotarget.3320

Figure 1. WIP1 silencing in salivary adenoid cystic carcinoma cells.

Figure 1

(A), ACC-M and ACC-2 salivary adenoid cystic carcinoma cells were transfected with WIP1-shRNA1, WIP2-shRNA2, or control shRNA using Lipofectamine LTX with plus and then photographed under an inverted fluorescence microscope (×100). (B), Western blotting analysis of WIP1 silencing by WIP1-shRNA1 and WIP2-shRNA in both ACC-M and ACC-2 cells. ß-actin loading control is also shown. Representative of three independent experiments was shown. (C), Transcription levels of WIP1 silencing by WIP1-shRNA1 and WIP2-shRNA in both ACC-M and ACC-2 cells, relative to GAPDH, determined by quantitative RT-PCR. Error bars represent the mean ± SD of triplicate experiments.