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. 2015 Mar 5;6(11):9031–9044. doi: 10.18632/oncotarget.3320

Figure 2. WIP1 silencing inhibits ACC-M cells migration and invasion.

Figure 2

(A and B), Migration (A) and invasion (B) assays in ACC-M cells. Representative images of migrated and invaded cells were shown under inverted microscopy. The mean was derived from cell counts of 5 fields, and each experiment was repeated 3 times. (C), Western blotting analysis of MMP-9, VEGF-C, MMP-2, MMP-13, and VEGF-D in WIP1-shRNA1 ACC-M cells. ß-actin loading control is also shown. Representative of three independent experiments was shown. (D), Transcription levels of WIP1, MMP-9, VEGF-C, MMP-2, MMP-13, and VEGF-D in WIP1-silenced ACC-M cells, relative to GAPDH, determined by quantitative RT-PCR. Error bars represent the mean ± SD of triplicate experiments.